DIAGNOSIS OF PARASITIC INFECTIONS EBOOK DOWNLOAD!
Approach to Parasitic Infections - Etiology, pathophysiology, symptoms, signs, diagnosis & prognosis from the MSD Manuals - Medical Professional Version. Rev Sci Tech. Sep;9(3) Diagnosis of parasitic disease. Ambrosio RE(1), de Waal DT. Author information: (1)Molecular Biology Section, Veterinary. Blood: Testing is used to identify the various stages of blood parasites and is routinely applied to diagnose malaria, theileriosis, babesiosis, anaplasmosis, ehrlichiosis, trypanosomiasis and most types of filariasis (14). Trypanosoma can also be diagnosed with wet smears.
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Serology-based diagnosis tools diagnosis of parasitic infections be divided into two categories: Although the ease of use and turnaround times for serologic assays are similar to microscopy, serology-based assays are more sensitive and specific. It becomes important for individuals whose blood smears do not permit identification of the parasite e.
Classifying an infected asymptomatic patient as negative could lead to transmission of diagnosis of parasitic infections parasite during blood transfusions or organ transplants.
In the case of Fasciola infection, serology tests have also been shown to be useful in the confirmation of chronic fascioliasis when egg production is low or sporadic [ ]. Finally, having these tests readily available allows for the monitoring of parasite clearance following therapy.
In the past, the method has been applied to the study of malaria [ 32 ], fasciolosis [ ], schistosomiasis reviewed in [ ]and taeniasis [ ]. However, this technique is subjected to the same drawbacks as most serology-based tests. Antibodies raised against a peptide from one parasite protein may cross-react with proteins from other species.
Moreover, antibodies raised against a peptide may diagnosis of parasitic infections in some assays but not in others and some regions of a peptide may be more immunogenic than others.
Diagnosis of parasitic disease.
In the dot-ELISA, the plastic plate is replaced by a nitrocellulose or other paper membrane onto which a small amount of sample volume is applied. The choice diagnosis of parasitic infections binding matrix greatly improved the specificity and sensitivity of the assay by reducing the binding of nonspecific proteins usually observed when plastic binding matrixes are used.
The principle is similar to the immunoblot. The dotted membrane is incubated first with diagnosis of parasitic infections antigen-specific antibody followed by an enzyme-conjugated anti-antibody.
The addition of a precipitable, chromogenic substrate causes the formation of a colored dot on the membrane which can be visually read [ 2 ].
CDC - Parasites - Resources - Diagnosis
The benefits of this technique include its ease of use, its rapidity, and the ease of result interpretation. It diagnosis of parasitic infections fast, and cost-effective and more importantly can be used in the field e.
For all these reasons, the Dot-ELISA has been and still is extensively used in the detection of human and animal parasitic diseases, including amebiasis, babesiosis, fascioliasis, cutaneous and visceral leishmaniasis, cysticercosis, diagnosis of parasitic infections, malaria, schistosomiasis, toxocariasis, toxoplasmosis, trichinosis, and trypanosomiasis all reviewed in [ 3 ].
In the last few years, published studies have demonstrated the use of the dot-ELISA for the detection of Fasciola gigantica [ ], Haemonchus contortus [ ], Theileria equi [ ], Trypanosoma cruzi [ ], and Trypanosoma brucei [ 34 ].
Diagnosis of Parasitic Diseases: Old and New Approaches
In the latter study the researchers were able diagnosis of parasitic infections demonstrate that the dot-ELISA had better sensitivity and specificity than the ELISA in the detection of antineurofilament and antigalactocerebrosides antibodies in cerebrospinal fluid of subjects infected with African trypanosomes.
They attributed the greater sensitivity and specificity of the dot-ELISA to the use of the nitrocellulose membrane and showed that their assay was successfully reproducible in the field. Rapid Antigen Detection System RDTS Rapid antigen detection tests RDTs based on immunochromatographic antigen detection have been implemented in many diagnostic laboratories as an adjunct to microscopy for the diagnosis of malaria.
RDTs consist of capturing soluble proteins by complexing them with capture antibodies embedded on a nitrocellulose strip. A drop of blood sample is applied to the strip and eluted from the nitrocellulose strip by the addition of a few drops of diagnosis of parasitic infections containing a labeled antibody.
The antigen-antibody complex can then be visualized directly from the membrane [ 4 ]. Since the appearance of the first RDTs in the s, major improvements have been made to the technique, making the use of RDTs diagnosis of parasitic infections rural endemic regions feasible.
RDTs are useful in the identification of P.
In addition, they are useless at detecting very low-density infections. PCR-based approaches remain the tool of choice in that situation.
- Diagnosis of parasitic disease.
- Approach to Parasitic Infections - Infectious Diseases - MSD Manual Professional Edition
- McGraw-Hill MedicalError | AccessMedicine | McGraw-Hill Medical
- Parasitic Infections
- Diagnosis of Parasitic Diseases